Fig. 4

Comparison of the effectiveness of monotherapy and combination therapy. A Schematic diagram of recombinant adeno-associated vectors. B mRNA expression levels of osteogenic genes Ocn, Opg (left) and osteoclast genes Rankl, Trap (right) after AAV-mediated gene therapy. C TRAP immunohistochemical staining and quantification. D Von kossa staining and calcification quantification results of knee joint. ***P < 0.001, **P < 0.01, and *P < 0.05