Fig. 1

Elevated expression of miR-182-5p in colorectal cancer (CRC) tissues and cell lines. A, C Hierarchical clustering heatmaps display the differential expression of miRNAs in CRC vs. normal tissues, derived from RNA sequencing data in the GEO database. Upregulated miRNAs are shown in red, downregulated ones in blue, with miR-182-5p highlighted by a red line. B, D Bioinformatics analysis revealing the expression pattern of miR-182-5p in CRC. E Comparative analysis of miR-182-5p expression levels between CRC tissues and normal tissues (control). F qRT-PCR analysis of miR-182-5p expression in various CRC cell lines (HT29, SW620, SW480, LOVO, and HCT116) vs. the normal cell line FHC. Data are presented as mean ± SD; **P < 0.01, comparing CRC groups to the FHC group