Fig. 5

NCALD downregulation triggers the activation of Wnt/β-catenin signalling. A, B Expression levels of Wnt/β-catenin signalling-related proteins (Wnt3a, GSK-3β, and β-catenin) in CRC tissues were analyzed using qRT-PCR and Western blot. **P < 0.01 vs. control group. C, D Effects of NCALD overexpression and knockdown on the expression of Wnt3a, GSK-3β, and β-catenin at both mRNA and protein levels were analyzed in CRC tissues, with GAPDH used as an internal control. **P < 0.01 for the over-NCALD group vs. control group; ^##P < 0.01 for the sh-NCALD group vs. control group. Data are presented as mean ± SD